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Enterobacteria identification and detection of diarrheagenic Escherichia coli in a Port Complex BJM
Costa,Clarissa Frota Macatrão; Monteiro Neto,Valério; Santos,Bruno Rafael de Carvalho; Costa,Bruno Rafael Rabelo; Azevedo,Alexandre; Serra,Josilene Lima; Mendes,Hermínio Benítez Rabello; Nascimento,Adenilde Ribeiro; Mendes,Mariana Bonfim Pinto; Kuppinger,Oliver.
The Port Complex of Maranhão (PCM) is the second largest port complex in Brazil, receiving ships with large volumes of ballast water. To evaluate the microbiological quality of its waters, physicochemical parameters (pH and salinity), the number of coliforms (thermotolerants and totals), and the presence of enterobacterias and diarrheagenic Escherichia coli strains were analyzed. In order to identify the presence of E. coli virulence genes target regions of the stx, elt, est, aggR, CVD432, ipaH and eae nucleotide sequences were studied. The presence of totals and thermotolerants coliforms were positive. Analyzing the salinity parameter, a significant increase in total coliforms was observed during the rainy season. We identified the species Escherichia...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microbiological analysis; Coliforms; Enterobacterias; Ballast water; Escherichia coli.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300026
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Identities among actin-encoding cDNAs of the Nile tilapia (Oreochromis niloticus) and other eukaryote species revealed by nucleotide and amino acid sequence analyses Genet. Mol. Biol.
Poletto,Andréia B.; Wasko,Adriane P.; Oliveira,Claudio; Azevedo,Alexandre; Carvalho,Robson F.; Silva,Maeli Dal Pai; Foresti,Fausto; Martins,Cesar.
Actin-encoding cDNAs of Nile tilapia (Oreochromis niloticus) were isolated by RT-PCR using total RNA samples of different tissues and further characterized by nucleotide sequencing and in silico amino acid (aa) sequence analysis. Comparisons among the actin gene sequences of O. niloticus and those of other species evidenced that the isolated genes present a high similarity to other fish and other vertebrate actin genes. The highest nucleotide resemblance was observed between O. niloticus and O. mossambicus a-actin and b-actin genes. Analysis of the predicted aa sequences revealed two distinct types of cytoplasmic actins, one cardiac muscle actin type and one skeletal muscle actin type that were expressed in different tissues of Nile tilapia. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Actin; Expression pattern; Nile tilapia; Oreochromis niloticus.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200031
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